Electrochemical sensors are devices able to determine the concentration, through direct electrical measurements, of molecules and biomolecules in solutions.

The miniaturization to a size below the 1 um in small-size arrays of nanoelectrodes (NEA), offers advantages in terms of increased sensitivity and compactness.

NEAs of nanoholes on a thin film of polycarbonate (PC) deposited on boron-doped diamond (BDD) macroelectrodes has successfully fabricated by thermal nanoimprint lithography (TNIL), with a high reliability and reproducibilioty.

The device has employed for the detection of, gliadin protein fragments physisorbed

on the polycarbonate surface of NEAs by immuno-indirect assay using a secondary

antibody labelled with horseradish peroxidase (HRP), in the range of concentration of 0.5–10 g/mL, by cyclic voltammetry.The fabrication of high-resolution nanostructures allows low-cost production, and possibly the scale up of NEAs-based electrochemical sensing platform to monitor biochemical molecules for both food and biomedical applications.